D. M. Brown, D. A. Phillips, D. C. Garcia, A. Arce, T. J. Lucci, J. P. Davies Jr, J. Mangini, K. A. Rhea, C. B. Bernhards, J. R. Biondo, S. M. Blum, S. D. Cole, J. A. Lee, M. S. Lee, N. D. McDonald, B. Wang, D. L. Perdue, W. Thavarajah, A. S. Karim, M. W. Lux, M. C. Jewett, A. E. Miklos, J. B. Lucks. ACS Synthetic Biology (2025). doi: 10.1021/acssynbio.4c00703
Links: Journal, BioRxiv
About
We investigated scalable manufacturing strategies for cell-free synthetic biology biosensors by implementing semi-automated assembly using the Opentrons OT-2 platform. Comparing manual and robotic workflows across colorimetric (LacZ) and fluorescent (GFP) assays, we demonstrated reliable 384-well production of fluoride-sensing biosensors with performance comparable to expected detection outcomes.
Leave a Reply