Adam Silverman’s paper, “Deconstructing Cell-Free Extract Preparation for in Vitro Activation of Transcriptional Genetic Circuitry,” is now published in ACS Synthetic Biology!
The manuscript, a collaboration with the Jewett lab, uncovers the relationship behind the methods for producing bacterial extracts for cell-free protein synthesis and the extracts’ protein productivity in vitro. Adam’s work finds that the method used to create extract matters a lot, particularly for proteins expressed using native bacterial transcriptional machinery! Also included: a very useful and detailed technical note to help other labs adopt cell-free protein synthesis techniques for their own.
Congrats Adam!
Lucks Laboratory 